Review





Similar Products

tlr3  (Proteintech)
93
Proteintech tlr3
Tlr3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tlr3/product/Proteintech
Average 93 stars, based on 1 article reviews
tlr3 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
anti tlr3  (Proteintech)
93
Proteintech anti tlr3
Anti Tlr3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti tlr3/product/Proteintech
Average 93 stars, based on 1 article reviews
anti tlr3 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
antibodies against tlr3  (ABclonal Biotechnology)
90
ABclonal Biotechnology antibodies against tlr3
Antibodies Against Tlr3, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies against tlr3/product/ABclonal Biotechnology
Average 90 stars, based on 1 article reviews
antibodies against tlr3 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
anti tlr3  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology anti tlr3
Anti Tlr3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti tlr3/product/Santa Cruz Biotechnology
Average 94 stars, based on 1 article reviews
anti tlr3 - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
tlr3 antibody  (Thermo Fisher)
90
Thermo Fisher tlr3 antibody
Tat interacts with TLR7, and TLR7 activation induces endolysosome damage. (A) Using biotin‐Tat as a bait, TLR7, but not <t>TLR3,</t> TLR8, or TLR9, was pulled down from U87MG cell lysates. (B) Using a biotinylated anti‐TLR7 antibody as bait to capture TLR7 from U87MG cell lysate and then incubate with Tat or mutant Tat, Tat, but not mutant‐Tat, was detected in the precipitates. A biotinylated isotype IgG was served as a negative control. (C) TLR7‐RFP colocalized with Tat‐FITC (blue) with a Pearson's correlation coefficient of 0.42. TLR7‐RFP colocalized with endolysosomes (Lysotracker, green) with a Pearson's correlation coefficient of 0.4 in human astrocytes ( n = 3). Scale bar = 10 μm. (D) TLR7 agonist R837 (1–10 μg/mL) induced endolysosome de‐acidification, as indicated by a decreased Green/Deep Red fluorescence ratio, scale bar =10 μm ( n = 3) . (E, F) TLR7 agonist R837 (1–10 μg/mL) significantly increased galectin‐3 puncta formation for 2 h (E) and 24 h (F) post‐treatment, scale bar =10 μm ( n = 5). Statistics: N = Independent replicates. One‐way ANOVA followed by Tukey's post hoc test.
Tlr3 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tlr3 antibody/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
tlr3 antibody - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
primary rabbit polyclonal antibodies against tlr3  (Biorbyt)
94
Biorbyt primary rabbit polyclonal antibodies against tlr3
Tat interacts with TLR7, and TLR7 activation induces endolysosome damage. (A) Using biotin‐Tat as a bait, TLR7, but not <t>TLR3,</t> TLR8, or TLR9, was pulled down from U87MG cell lysates. (B) Using a biotinylated anti‐TLR7 antibody as bait to capture TLR7 from U87MG cell lysate and then incubate with Tat or mutant Tat, Tat, but not mutant‐Tat, was detected in the precipitates. A biotinylated isotype IgG was served as a negative control. (C) TLR7‐RFP colocalized with Tat‐FITC (blue) with a Pearson's correlation coefficient of 0.42. TLR7‐RFP colocalized with endolysosomes (Lysotracker, green) with a Pearson's correlation coefficient of 0.4 in human astrocytes ( n = 3). Scale bar = 10 μm. (D) TLR7 agonist R837 (1–10 μg/mL) induced endolysosome de‐acidification, as indicated by a decreased Green/Deep Red fluorescence ratio, scale bar =10 μm ( n = 3) . (E, F) TLR7 agonist R837 (1–10 μg/mL) significantly increased galectin‐3 puncta formation for 2 h (E) and 24 h (F) post‐treatment, scale bar =10 μm ( n = 5). Statistics: N = Independent replicates. One‐way ANOVA followed by Tukey's post hoc test.
Primary Rabbit Polyclonal Antibodies Against Tlr3, supplied by Biorbyt, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary rabbit polyclonal antibodies against tlr3/product/Biorbyt
Average 94 stars, based on 1 article reviews
primary rabbit polyclonal antibodies against tlr3 - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
sc 32232  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology sc 32232
Tat interacts with TLR7, and TLR7 activation induces endolysosome damage. (A) Using biotin‐Tat as a bait, TLR7, but not <t>TLR3,</t> TLR8, or TLR9, was pulled down from U87MG cell lysates. (B) Using a biotinylated anti‐TLR7 antibody as bait to capture TLR7 from U87MG cell lysate and then incubate with Tat or mutant Tat, Tat, but not mutant‐Tat, was detected in the precipitates. A biotinylated isotype IgG was served as a negative control. (C) TLR7‐RFP colocalized with Tat‐FITC (blue) with a Pearson's correlation coefficient of 0.42. TLR7‐RFP colocalized with endolysosomes (Lysotracker, green) with a Pearson's correlation coefficient of 0.4 in human astrocytes ( n = 3). Scale bar = 10 μm. (D) TLR7 agonist R837 (1–10 μg/mL) induced endolysosome de‐acidification, as indicated by a decreased Green/Deep Red fluorescence ratio, scale bar =10 μm ( n = 3) . (E, F) TLR7 agonist R837 (1–10 μg/mL) significantly increased galectin‐3 puncta formation for 2 h (E) and 24 h (F) post‐treatment, scale bar =10 μm ( n = 5). Statistics: N = Independent replicates. One‐way ANOVA followed by Tukey's post hoc test.
Sc 32232, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sc 32232/product/Santa Cruz Biotechnology
Average 94 stars, based on 1 article reviews
sc 32232 - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier

Image Search Results


Tat interacts with TLR7, and TLR7 activation induces endolysosome damage. (A) Using biotin‐Tat as a bait, TLR7, but not TLR3, TLR8, or TLR9, was pulled down from U87MG cell lysates. (B) Using a biotinylated anti‐TLR7 antibody as bait to capture TLR7 from U87MG cell lysate and then incubate with Tat or mutant Tat, Tat, but not mutant‐Tat, was detected in the precipitates. A biotinylated isotype IgG was served as a negative control. (C) TLR7‐RFP colocalized with Tat‐FITC (blue) with a Pearson's correlation coefficient of 0.42. TLR7‐RFP colocalized with endolysosomes (Lysotracker, green) with a Pearson's correlation coefficient of 0.4 in human astrocytes ( n = 3). Scale bar = 10 μm. (D) TLR7 agonist R837 (1–10 μg/mL) induced endolysosome de‐acidification, as indicated by a decreased Green/Deep Red fluorescence ratio, scale bar =10 μm ( n = 3) . (E, F) TLR7 agonist R837 (1–10 μg/mL) significantly increased galectin‐3 puncta formation for 2 h (E) and 24 h (F) post‐treatment, scale bar =10 μm ( n = 5). Statistics: N = Independent replicates. One‐way ANOVA followed by Tukey's post hoc test.

Journal: Aging Cell

Article Title: TLR7 Mediates HIV ‐1 Tat‐Induced Cellular Senescence in Human Astrocytes

doi: 10.1111/acel.70086

Figure Lengend Snippet: Tat interacts with TLR7, and TLR7 activation induces endolysosome damage. (A) Using biotin‐Tat as a bait, TLR7, but not TLR3, TLR8, or TLR9, was pulled down from U87MG cell lysates. (B) Using a biotinylated anti‐TLR7 antibody as bait to capture TLR7 from U87MG cell lysate and then incubate with Tat or mutant Tat, Tat, but not mutant‐Tat, was detected in the precipitates. A biotinylated isotype IgG was served as a negative control. (C) TLR7‐RFP colocalized with Tat‐FITC (blue) with a Pearson's correlation coefficient of 0.42. TLR7‐RFP colocalized with endolysosomes (Lysotracker, green) with a Pearson's correlation coefficient of 0.4 in human astrocytes ( n = 3). Scale bar = 10 μm. (D) TLR7 agonist R837 (1–10 μg/mL) induced endolysosome de‐acidification, as indicated by a decreased Green/Deep Red fluorescence ratio, scale bar =10 μm ( n = 3) . (E, F) TLR7 agonist R837 (1–10 μg/mL) significantly increased galectin‐3 puncta formation for 2 h (E) and 24 h (F) post‐treatment, scale bar =10 μm ( n = 5). Statistics: N = Independent replicates. One‐way ANOVA followed by Tukey's post hoc test.

Article Snippet: The following antibodies were used: HIV‐1 Tat antibody (ImmunoDX, cat#1302, dilution 1:1000 and/or Santa Cruz, cat#sc‐65,913, dilution 1:250), TLR7 antibody (ABonline, cat#ABIN3021247, dilution 1:250), TLR3 antibody (Thermo Fisher, cat#PA5‐20183, dilution 1:1000), TLR8 antibody (Invitrogen, cat# PA5‐20056, dilution 1:1000), TLR9 antibody (Thermo Fisher, cat# PA5‐20203, dilution 1:1000), p16‐INK4A (Proteintech, cat#10883‐1‐AP, dilution 1:500), and p21 CIP1 (cell signaling, cat#2947S, dilution 1:500).

Techniques: Activation Assay, Mutagenesis, Negative Control, Fluorescence